James Mackie
James Mackie may refer to:
- James Stuart Mackie (1860–1949), Canadian businessman and politician
- Jamie Mackie (born 1985), English born footballer
- Jim Mackie (1889–1917), Australian rules footballer
- James Mackie (EastEnders), fictional character
- James Mackie (MP) (1821–1867), British member of parliament for Kirkcudbright
- James Mackie (moderator), Church of Scotland minister and historian
- James Mackie (died 1744), commander of the Royal Navy fire-ship HMS Anne Galley from 1742 to 1744
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